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DG (12.18 and 36.54mg/kg) and venlafaxine (VLX) administration significantly increased hedonic behavior in mice in the SPT. Sucrose preference (SPT) and tail suspension (TST) tests were then performed to evaluate depressive-like behaviors in mice. Mice were randomly divided into five groups and injected daily until testing with 0.9% NaCl solution, one of three doses of DG (4.06, 12.18, and 36.54mg/kg), or venlafaxine. Here, we investigated the antidepressant-like effects of diterpene ginkgolides (DG), an important class of constituents in GBE, and studied their effects in the mouse hippocampus using a GC-MS-based metabolomics approach. However, because GBE contain many constituents, the mechanisms underlying this suggested antidepressant activity are unclear. Electronic address: biloba extract (GBE), including EGb-761, have been suggested to have antidepressant activity based on previous behavioral and biochemical analyses. 6 Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China Chongqing Key Laboratory of Neurobiology, Chongqing, China Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing, China Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing, China.Electronic address: 1 Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China Chongqing Key Laboratory of Neurobiology, Chongqing, China Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing, China Department of Neurology, The Inner Mongolia Autonomous Region people's Hospital, Hohhot, Inner Mongolia, China. 5 Chongqing Key Laboratory of Neurobiology, Chongqing, China Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing, China.4 Chongqing Key Laboratory of Neurobiology, Chongqing, China Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing, China Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing, China.3 Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China Chongqing Key Laboratory of Neurobiology, Chongqing, China Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing, China.2 Chongqing Key Laboratory of Neurobiology, Chongqing, China Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing, China Key Laboratory of Laboratory Medical Diagnostics of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, China.1 Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China Chongqing Key Laboratory of Neurobiology, Chongqing, China Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing, China Department of Neurology, The Inner Mongolia Autonomous Region people's Hospital, Hohhot, Inner Mongolia, China.